The expression of leptin in oral wound healing in diabetes mellitus: An experimental study
Abstract
Objective: The present work evaluated histologically and immunohistochemically, the expression of leptin during healing of the incisional oral mucosal wound in diabetic rats as compared to healthy rats.
Methods: Twenty-four, adult male Sprague–Dawley rats weighing on average150-200 gm were allocated equally into two groups: Group I (Control) and Group II (diabetic). Diabetes was induced by a single intraperitoneal injection of Streptozotocin STZ dissolved in distilled water. Each animal received experimental incision in buccal mucosa and sutured, the specimens were collected from the buccal mucosa of each animal at intervals of 7, 14,21 days and routinely processed for H&E and immunohistochemical staining for leptin. All measurement data were calculated as mean ± standard deviation.
Results: Leptin expression was observed in the epithelium and in the vascular endothelial cells in both groups. In both the control and diabetic groups, the expression of leptin was significantly increased with time, there was extreme highly significant increase in the control group than in diabetic group after 7, 14 and 21 days (p = 0.000).
Conclusion: The results of the present study suggested that leptin may promote wound healing in rat’s normal oral mucosa more than in diabetic. Further studies are needed to clarify the exact molecular mechanisms of leptin’s effects on wound healing and to determine the usefulness of leptin as a treatment to promote wound healing in the oral mucosa in diabetic and non-diabetic patients.
Keywords:
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).