Protective potential of Vitamin E against methylphenidate-induced male gonadal changes in albino rats

Abstract

Objective: Attention deficit hyperactivity disorder ranks among the top neuropsychiatric disorder of childhood and adolescents. Methylphenidate (MPH) is the most frequently used pharmacologic agent to treat this condition. Its long-term use has been associated with many unwanted and adverse effects on many organs including male gonads, but so far no study has been done to find out a protective agent. This study investigated the protective potential of Vitamin E (Vit E) against the microscopic and morphometric alterations in male gonads induced by MPH, using albino rats.


Materials and Methods: Adult male albino rats were assigned into three equal groups including one control and two experimental groups. Experimental groups administered with MPH (10 mg/kg) and MPH (10 mg/kg) + Vit E orally (50 mg/kg), daily for 40 days. Testes of the sacrificed animals were removed, processed, and stained with hematoxylin and eosin for examining the microscopic and morphometric alterations and protective potential of Vit E. Data were analyzed using ANOVA.


Results: Experimental animals treated with MPH showed a significant decrease in the diameter of seminiferous tubules (296.86 ± 14.70 μm) and height of germinal epithelium (51.73 ± 3.15 μm) with a corresponding gain in the thickness of the interstitium (47.05 ± 4.94 μm). Animals treated with MPH + Vit E did not reveal any significant testicular microscopic changes and seminiferous tubular alterations induced by MPH.


Conclusion: Vit E demonstrated a protective potential against the adverse changes induced by MPH in the male gonads in albino rats.

Keywords:

Attention deficit hyperactive disorder diameter of semineferous tubules height of germinal epithelium methylphenidate reactive oxygen species thickness of interstitial spaces Vitamin E
Iqbal, S., Hameed, U., Hasan, B., Islam, Z. ul, Ahmed, M., & Brohi, A. H. (2019). Protective potential of Vitamin E against methylphenidate-induced male gonadal changes in albino rats. International Journal of Health Sciences, 13(3). Retrieved from https://pub.qu.edu.sa/index.php/journal/article/view/3777
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Author Biographies

Uzma Hameed, Liaquat national hospital and medical college

assistant professor

department of anatomy

Batool Hasan, Liaquat national hospital and medical college

lecturer

department of anatomy

Zia ul Islam, Liaquat national hospital and medical college

professor

head of department of anatomy

Masood Ahmed, Liaquat national hospital and medical college

professor 

department of anatomy

Aisha Hassan Brohi, Liaquat national hospital and medical college

lecturer

department of anatomy