Effect of bacillus bacterial extracts on the expression of major histocompatibility complex Class I and Class II receptors on MDA-MB 231 breast cancer cells

Bacterial extracts & MHC class I and class II receptors

Authors

  • Waleed Alabdulmonem Pathology Department, College of Medicine, Qassim University, Qassim

Keywords:

Bacillus bacterial extracts, breast cancer cells, major histocompatibility complexes Class I receptor, major histocompatibility complexes Class II receptor

Abstract

Objective: The expression of human leukocyte antigen (HLA)-ABC and HLA-DR is linked to the inflammatory disorders and this includes the development of breast cancer. This study was aimed to determine the effect of bacillus bacterial extracts on the expression of the major histocompatibility complexes (MHC) Class I and Class II receptors on breast cancer cells.

Methods: The expression of HLA-ABC and HLA-DR was assessed by flow cytometry and confocal microscope on the human breast tumor cell lines MDA-MB-231, which used a readily accessible model system. It is postulated that the HLA-ABC and HLA-DR receptors might be regulated by bacillus bacterial extracts designated (RA10, RA4, RA7, and RA16).

Results: It was observed that the treatment of cell line MDA-MB-231 with the RA10 resulted in an upregulation of the cell surface expression of the HLA-A,B,C receptors. It was observed that RA10 has the capacity to reduce the expression of HLA-A,B,C, it also shows a detectable degree of cytotoxicity when used at high concentrations. The data shows that the cell surface expression of HLA-ABC is higher than HLA-DR. No significant changes of HLA-DR expression was observed on MDA-MB-231 cell lines.

Conclusions: Improved understanding of the connection between HLA-ABC, HLA-DR, and bacterial extracts such as RA10 may lead to the development of drug design and therapies related to breast cancer condition in which these receptors are involved.

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Published

2019-12-25

How to Cite

Alabdulmonem, W. (2019). Effect of bacillus bacterial extracts on the expression of major histocompatibility complex Class I and Class II receptors on MDA-MB 231 breast cancer cells: Bacterial extracts & MHC class I and class II receptors. International Journal of Health Sciences, 14(1). Retrieved from https://pub.qu.edu.sa/index.php/journal/article/view/4529

Issue

Vol. 14 No. 1 (2020): Jan.-Feb.

Section

Original Paper

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