Frequency of UCP2 45-bp Ins/Del polymorphism in Saudi population from Jazan area and its association with autoimmune hypothyroidism UCP2 45-bp Ins/Del frequency in hypothyroidism

Authors

  • Yahia A. Kaabi
  • Abdullah S. Mansor Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, Jazan University, Jazan, Kingdom of Saudi Arabia
  • Ashwag S. Alfagih Endocrinology and Diabetes Center, King Fahad Central Hospital, Jazan, Kingdom of Saudi Arabia
  • Alhussain M. Hakami Endocrinology and Diabetes Center, King Fahad Central Hospital, Jazan, Kingdom of Saudi Arabia
  • Mohammed A. Summ Endocrinology and Diabetes Center, King Fahad Central Hospital, Jazan, Kingdom of Saudi Arabia
  • Yahia A. Mjery Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, Jazan University, Jazan, Kingdom of Saudi Arabia
  • Mona N. Alzughbi Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, Jazan University, Jazan, Kingdom of Saudi Arabia
  • Mahmoud M. Habibullah Dr

Keywords:

Autoimmune hypothyroidism, Saudi Arabia, uncoupling protein 2

Abstract

Objectives: Autoimmune hypothyroidism (AHT) is a common endocrine disorder. Although the exact cause of AHT is not yet understood, genetic factors may play a major role. Uncoupling protein 2 (UCP2) is a member of mitochondrial protein family involved in the regulation of cellular metabolism. An important functional polymorphism in the UCP2 gene, 45-bp insertion/deletion (ins/del) polymorphism, has been linked to certain clinical conditions. However, an association between the 45-bp ins/del polymorphism and AHT has not yet been established.

Methods: In this study, about 259 blood samples were collected from, patients with AHT and age-matched healthy control subjects. DNA was extracted for UCP2 45-bp ins/del polymorphisms genotyping, using a standard polymerase chain reaction technique. The distribution of different genotypes was determined in both groups and possible association with AHT was also assessed by logistic regression analysis using the Del/Del variant as a reference genotype.

Results: The frequency of the UCP2 45-bp ins/del polymorphism in the total study population was 49.04%, 40.15%, and 10.81% for Del/Del, Ins/Del, and Ins/Ins genotypes, respectively. The logistic regression analysis showed crude odds ratios (ORs), respectively, with their 95% confidence intervals (CIs) and P-values in codominant (Del/Ins) (OR = 1.53, CI = 0.89–2.60, P = 0.17), codominant (Ins/Ins) (OR = 0.75, CI = 0.34–1.74, P = 0.53), dominant (OR = 1.30, CI = 0.79–2.16, P = 0.37), and recessive (OR = 0.62, CI = 0.29–1.36, P = 0.30) inheritance models tested, where none of which were statistically significant.

Conclusion: Our data revealed the distribution of the UCP2 45-bp ins/del polymorphisms in Jazan area and confirmed the lack of association between these genetic variants and the development of AHT.

Author Biography

Yahia A. Kaabi

Associate Professor of Clinical Chemistry 

Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, Jazan

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Published

2020-06-29

How to Cite

Kaabi, Y. A. ., Mansor, A. S., Alfagih, A. S., Hakami, A. M. ., Summ, M. A., Mjery , Y. A., Alzughbi, M. N., & Habibullah, M. M. (2020). Frequency of UCP2 45-bp Ins/Del polymorphism in Saudi population from Jazan area and its association with autoimmune hypothyroidism UCP2 45-bp Ins/Del frequency in hypothyroidism. International Journal of Health Sciences, 14(4), 11–16. Retrieved from https://pub.qu.edu.sa/index.php/journal/article/view/4848

Issue

Vol. 14 No. 4 (2020): Jul-Aug

Section

Original Paper

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