Application of magnetic nanoparticles by comparing the absorbance and stabilization of biomolecules DNA-C, L by the electrophoretic detection
Abstract
Objectives: The project is based on extensive studies on applied nanoparticles in biology and medicine. This study was primarily designed to investigate the role of magnetic nanoparticles by comparing the absorbance and stabilization of DNA-C, L by the electrophoretic detection.
Methods: Fe3O4 magnetic nanoparticles (MNPs) with core/shell structure of silica coatings were synthesized by a chemical coprecipitation method. This work is done at 15 min times with multitimes, that is, 20 numbers and nanoparticles are provided regular with good morphology which was synthesized in 20 nm in size, and its structure was analyzed by tools such as EDX analysis. Next, 20 mg of the magnetic nanoparticles were coated with silica in a heterogeneous solution at 25 μg / ml of the solution of each of the mixed DNAs (ring, linear) in separate containers. Finally, 15 minutes later, DNA was adsorbed on the surface of the nanoparticles. The amount of this adsorption was injected by spectrophotometry (UV-Vis, ith 99% accuracy and optimized by the standard Tris.HCl buffer required to separate DNA from its pure solution (unabsorbed DNA)) and electrophoresis.
Results: The results showed that absorption and diffusion of DNA-C or L at the surface of nanoparticles were 95% and 85%, respectively (i.e., absorbance of DNA-C>DNA-L is) with rate of removing of on MNPs was >99%. Hence, after review, we received that a linkage of electrostatic bonding between nanoparticles and biomolecules was obtained, and the results of the EDX analysis confirmed this study.
Conclusion: In this project, nanocomposites containing magnetic nanoparticles were synthesized and their structure was identified by relevant analyzes. It was then used to stabilize the biomolecules, which yielded competitive results between the two types of DNA (linear and cyclic) at 85 and 95% adsorption, respectively.
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