Diagnosis of Sex Chromosome Disorders and Prenatal Diagnosis of Down Syndrome using

Abstract

Background : Thousands of infants are born each year with chromosomal abnormalities that severely impact physical and
mental development. Among common genetic disorders are Down syndrome (trisomy 21) and sex chromosomal disorders.
Objectives : Evaluation of guidelines used for prenatal diagnosis of Down syndrome (DS) as well as sex chromosomal
disorders including interphase Fluorescent In Situ Hyperidization (FISH) technique.
Methods : Enrolled cases were among those presenting to Genetics and Neonatology Units, Mansoura and Ain-Shams
University hospitals,(Egypt) during 2002 to 2004. These included: Groups 1 comprised fifty pregnant women presenting for
genetic counseling. They were subjected to complete history analysis, ultrasound examination in addition to triple screening test
(for alpha feto protein (AFP), human chorionic goandotrophin (HCG) and unconjugated esteriol (E2). Results were confirmed by
doing routine karyogram on cultured amniotic fluid. Groups 2 comprised suspected cases with sex chromosomal disorders
including neonates with ambiguous genitalia (64 cases) and adults with primary amenorrhea (69 cases) or infertility (38 cases).
They were subjected to a diagnostic workup including
Results : Among the pregnant women group, seven were found to be at a high risk of having DS fetuses including 3 cases with
a history of affected off-springs, 2 cases with age above 35 years, and 2 cases with high triple test. Only one case had positive
trisomy 21 on interphase FISH confirmed by karyogram on cultured amniotic cells. The other 6 ladies had normal FISH
confirmed by karyograms. Regarding the other group, 5 cases out of the 9 females were proved to be feminized males, one
proved mosaic turner, one proved mixed gonadal dysgenesis and 2 normal females. On the other hand one out of three males
were proved to be verilized female while the other one was a male with incomplete testicular feminization and the last one was a
male with infertility diagnosed as Klinefelter syndrome at the age of 26 years.
Conclusion : Interphase FISH is a rapid, accurate and very sensitive method in sex chromosom and autosomal abnormalities.
It adds to the diagnostic utility of routine cytogenetics and its use on interphase nuclei overcomes the difficulty of conventional
cytogenetics. It could be used in the prenatal diagnosis of DS in addition to ultrasonography, and triple test.
Settin, A., Abu-Saif, I. S., El-Baz, R., Dowaidar, M., Abu-Al Kasim, R., & Shabana, S. (2007). Diagnosis of Sex Chromosome Disorders and Prenatal Diagnosis of Down Syndrome using. International Journal of Health Sciences, 1(2). Retrieved from https://pub.qu.edu.sa/index.php/journal/article/view/84
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