Restoration of renal hemodynamics and functions by nigella sativa administration in dinitrophenol-induced hypoxia in rats animal model
Abstract
Objective: Hypoxia is one of the principal causes of renal diseases. This study aimed to evaluate effects of Nigella sativa (N. sativa) on dinitrophenol (DNP) - induced hypoxia renal damage in rats.
Methods: Forty adult male rats were incorporated in this study. The rats were divided into 4 groups: Control group, N. sativa group, DNP hypoxic group and DNP+N. sativa group receiving N. sativa (400 mg/kg body weight). Serum and renal tissue erythropoietin (EPO) hormone, hypoxia inducible factor-2α (HIF-2α) levels were measured. Renal oxidative stress biomarkers, inflammatory biomarkers, renal hemodynamics and histopathological examination were evaluated.
Results: Administration of N. sativa highly significantly normalize serum EPO level, hypoxia inducible factor-2α (P<0.001 for each) in DNP+N. sativa treated rats as compared to DNP hypoxic rats. Also, it highly significantly improved renal oxidative stress evident by decreased renal tissues MDA and increased SOD, total thiol, and CAT activity (P<0.001 for each). Also, highly significantly decline of renal ICAM-1, MPO, IL-6 was observed in DNP+N. sativa rats (P<0.001 for each). Improvement of renal hemodynamics and kidney functions were also found after N. sativa administration (with P value < 0.001 for all parameters). In addition, N. sativa treatment reduced renal histopathological changes of DNP+N. sativa group. Our results were statistically analyzed using the Prism software package (graph pad version 8.0).
Conclusions: N. sativa has an alleviating effect on DNP-induced hypoxia renal damage and can restore kidney functions in rats’ animal model. These effects were via anti-oxidant, anti-inflammatory and hemodynamic mechanisms.
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