Growth plate changes associated with Hypothyroidism amongst the pre and postnatal rats

Abstract

Objectives: This study is intended to examine the effect of hypothyroidism and thyroxin replacement on axial and appendicular skeletal growth, growth plate thickness and to observe associated microscopic changes within the growth plates.

Methodology: Experimental albino rats were developed with carbimazole and carbimazole plus thyroxin. Animals were administered with these drugs throughout pregnancy (prenatally) and for a period of six weeks, postnatally. At the end of the experiment the animals were sacrificed, measured and processed to demonstrate the bony and cartilaginous parts. Ulna and tibia of both sides were removed and processed for light microscopy and growth plate measurement.

Results: At the end of the experiment, reduction in the crown rump length was observed in the carbimazole treated animals. It was 8.77%, 13.26% and 7.25% in the prenatal, two weeks and six weeks age group animals respectively. In carbimazole plus thyroxin treated animals, this reduction was 3.22%, 2.94% and 3.42%, when compared to their age matched controls. Reduction in the thickness of the Epiphyseal growth plate (EGP) was 16.89% and 12.80% in the ulna of the two and six weeks age group  and 18.06 % and 15.65% in the tibia of the these animals respectively. The carbimazole plus thyroxin treated animals showed an increase in the thickness of EGP as compared to their age -matched controls though the crown rump length of these animals was less than the controls. Prenatally treated hypothyroid rats showed disrupted growth plates without any well-formed microscopic zones.

Conclusion: The results of this study showed that the pre and postnatally, carbimazole induced hypothyroidism and its replacement therapy affected the axial and appendicular skeletal growth.  Proximal limb bones of the prenatally induced hypothyroid animals showed the greatest skeletal change in this study.

Key words: Growth plate, Hypothyroidism, skeletal growth, Prenatal growth, Thyroid dysfunction

Shaikh, M. A., Naeem, Z., Alshahat, A. A., Shaikh, F. A., & Arif, S. (2013). Growth plate changes associated with Hypothyroidism amongst the pre and postnatal rats. International Journal of Health Sciences, 7(1). Retrieved from https://pub.qu.edu.sa/index.php/journal/article/view/504
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Author Biographies

Masood Ahmed Shaikh, Professor, Department of Anatomy and Histology, College of Medicine, Qassim University, KSA
Professor, Department of Anatomy and Histology,
Zahid Naeem, Department of Community medicine, College of medicine, Qassim University, Saudiarabia
Professor, Department of Community medicine, College of medicine, Qassim University, Saudiarabia
Abd Alrahman Alshahat, Department of Anatomy, College of Medicine, Cairo University, Egypt

Lecturer, Department of Anatomy, College of Medicine, Cairo University, Egypt

Farooque Ahmed Shaikh
- Lecturer, Department of Anatomy, College of Medicine, King Saud University, Riyadh, Saudiarabia

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