N/A

Objective: Content-based image retrieval (CBIR) is the most suitable and alternative method for older text searches that use keywords. This article aims to improve feature extraction as well as matching techniques designed for more accurate and precise CBIR systems, especially for brain scan images associated with various brain diseases and abnormalities. Tests should be described at an appropriate success rate. Methods: Various methods of producing medical images are discussed, and examples of biological applications are given. The discussion emphasizes as an introduction to CBIR the new method of echo-planar imaging, which is fully described. We have done here many methods related to digital image processing and we had developed a code for retrieving everything automatically. This application has been developed in Matlab software. Results: Testing the correctness and effectiveness of the system evolved becomes more important when the system is going to be used in real-time and more when it is for humankind, i.e., medical diagnosis. Nowadays, our science and technology areas as develop as we can say that we have such advanced medical equipment so that our thought and program can be capable that it is giving us useful results. Determining if whether the two images are identical or not, it depends on the point of view of the person. Conclusions: In this paper, the outcome of feature extraction and matching by setting cutoff limit and threshold is pretty promising. Further studies can be done apart from computed tomography scans for a more generalized CBIR system.

Objective: This study was undertaken to evaluate the formulation of the microspheres for antiulcer drug using natural polymer Acacia nilotica gum. Materials and Methods: All parameters evaluated A. nilotica gum, aqueous solution of purified gum was used for chemical characterization, organoleptic character, flow properties, pH, particle size determination, solubility, viscosity, surface tension, infrared spectroscopy, etc. The microspheres of famotidine were prepared by ionotropic gelation technique using cross-linking solution of aluminum chloride, barium chloride, and calcium chloride. Results: Micrometric evaluation was performed of natural polymer microspheres. The micrometric parameters such as bulk density, bulkiness, compressibility index Hausner’s ratio, and angle of repose better flow and good packaging properties (bulk density F5±0.02) formulation F1 showed maximum tapped density 0.06±0.04. The concentration was changed of A. nilotica gum so formulation F5 given maximum percentage yield of microspheres. Particle size ranges 846.31–989.04 μm. Formulation F3 shows the smallest particle size and F5 shows the largest particle size. Swelling index for all the formulations was found in an excellent range from 114.2 to 144.4. Formulation F5 showed the highest swelling property. Highest drug content was found in formulation F1 ranged from 88.98% to 98.23%. In in vitro drug release, F1 showed maximum release of drug, whereas F6 showed minimum release of drug. Conclusions: Natural polymer A. nilotica gum was successfully used for the preparation of famotidine microparticulates. Formulation with A. nilotica gum was impacted to the particular size, surface morphology, swelling conduct, and in vitro drug release. Stability studies F1, F3, and F5 formulations showed no reduction in drug content and in percent release. In short, A. nilotica gum may be utilized during pharmaceutical dose frames by giving support to drug delivery system and avoiding side effects for the patients.

Objective: The objective of the study was to evaluate the relative occurrence of uterine corpus tumor-like lesions and to establish a clinicopathological correlation. Methods: A 5-year study was conducted on histopathologically diagnosed cases of tumor-like lesions of the uterine corpus. The lesions were classified according to the recent World Health Organization classification. Relevant clinical findings and histomorphologic details were noted and analyzed. Results: A total of 85 cases of tumor-like lesions were included in the study. Multiparous women (88.2%) were most commonly affected and endometrial polyp (89.4%) was the most common lesion. Endometrial polyp showed a definite predilection during the 3rd and 4th decades of life (56.6%) whereas Arias-Stella reaction was seen in pregnant women and squamous metaplasia was seen in peri and postmenopausal women. Patients with endometrial polyp presented mostly with heavy menstrual bleeding (HMB) (32%) and Arias-Stella reaction with HMB. Two cases of squamous metaplasia one presented with HMB and pain in the abdomen. Most of the endometrial polyps were single (90.8%) and sessile (68.4%), hyperplastic type (56.6%) and the adjacent endometrium were mostly atrophic (42.1%) or in proliferative phase (34.2%). The mean age in years of premenopausal women was 39.34 ± 5.01 and postmenopausal women were 62.66 ± 7.26. The mean size of the polyp, parity, number of polyps and association with leiomyoma was not significantly different between premenopausal and postmenopausal women. Adenomyosis was seen in 11 cases of the endometrial polyp. Conclusions: Although tumor-like lesions of the uterine corpus as the name suggests tend to mimic the tumors, certain clinical and histopathological features can help in making the accurate diagnosis and thus avoid unnecessary radical surgeries.

Objective: The objective of the study was to analyze the effect of intrathecal transplantation of autologous bone marrow-derived mononuclear cells (BMMNCs) in functional recovery of spinal cord injury (SCI) patients along with neurorehabilitation and to evaluate various factors influencing the outcome of cellular therapy. Methods: We conducted an open-label study including 180 sub-acute and chronic SCI patients. All patients received intrathecal autologous BMMNCs along with neurorehabilitation. 80–100 mL of bone marrow was aspirated and BMMNCs were obtained using density gradient separation. An average of 1.06 × 108 cells with 97% viability was administered through lumbar puncture immediately. After transplantation, all patients underwent neurorehabilitation. Patients were followed up after an average of 9 ± 7 months. They were assessed for functional symptomatic changes and the outcome measures used were functional independence measure (FIM) and walking index for SCI (WISCI). Results: Patients showed symptomatic improvement in sitting/standing balance, bed mobility, trunk stability, upper limb function, mobility, sensation, bowel/bladder functions, and activities of daily living with no serious adverse events. Scores on FIM and WISCI showed statistically significant improvement. On subgroup analysis, it was found that early intervention and more than one dose of BMMNCs demonstrate a better functional outcome. Younger patients demonstrated better improvements in functional independence. Both cervical and dorsolumbar levels of injury show significant improvements in motor and sensory deficits. Conclusions: Autologous BMMNC transplantation with neurorehabilitation is safe, effective, enhances functional recovery, and improves the quality of life of SCI patients in sub-acute and chronic stage.

Introduction: The major transcription factor, which modulates the epithelial-mesenchymal transition in different types of cancers, is known as TWIST oncogene. It binds to the promoter of E-cadherin and suppresses its transcription. The current study aims to assess the expression of TWIST protein in oral squamous cell carcinoma (OSCC), epithelial dysplasia (ED), and normal oral mucosa to verify whether such protein is useful as a marker in oral epithelium malignant transformation. Methods: Thirty-five paraffin-embedded tissue samples of oral lesions with ED and OSCC and five samples of normal oral mucosa were immuostained with anti‑TWIST antibody using the streptavidin peroxidase method. Results: TWIST expression was negative in all cases of normal oral mucosa, whereas all cases of ED and OSCC showed positive immunoreactivity to TWIST varied from weak to strong expression. In ED, there was a significant difference between severe dysplasia and the other two types (P = 0.03). TWIST expression had no significant relationship with the clinical parameters of OSSC clinical stage and grade (degree of differentiation). Only two cases of OSCC with lymph node metastasis showed strong nuclear TWIST expression. Intergroups assessment indicated a significant increase of TWIST expression in OSCC compared to ED (P = 0.000). Conclusion: A significant increase of TWIST expression in OSCC compared to ED may suggest its role in carcinogenesis, it may be a useful marker in malignant transformation of oral epithelium. Therefore, TWIST might be an important target for therapeutic approaches in patients with OSCC, which requires further investigations.

Objectives: While empagliflozin (25 mg) is used to treat type-2 diabetes mellitus patients with optimum renal functioning, its efficacy and safety in type-1 diabetes mellitus (T1DM) is not yet established. Therefore, this study aimed to compare insulin-treated T1DM patients’ (with adequate renal functioning) glycemic and blood pressure control between the 25 mg empagliflozin recipients and placebo recipients. Methods: Parallel-arm randomized controlled trials comparing the effect of daily administered 25 mg empagliflozin tablets in adjunct to insulin treatment with placebo and insulin treatment in T1DM patients with an estimated glomerular filtration rate of 45 mL/min/1.73 m2 or more were eligible for inclusion. Trials were searched in PubMed, EMBASE, SCOPUS, and CENTRAL with no restriction on date and language. Risk of bias of trials was assessed and mean and standard deviation of glycated hemoglobin (HbA1c, in %), systolic blood pressure (mmHg), and diastolic blood pressure (mmHg) at the end of the trial period were collected, and random-effects meta-analysis was done to estimate the weighted mean difference (WMD). The metaanalysis was done in Stata statistical software. This study was conducted in June 2019. Results: Three relatively small-sized trials published between 2015 and 2018 were eligible for review and analyses. The trials suffered from unclear risk of performance and detection bias. The HbA1c reduction favored the intervention group (WMD = −0.478, 95% confidence intervals = −0.766–−0.189, P = 0.001; I2 = 0%). The WMD of blood pressure (systolic and diastolic) did not vary between the treatment groups. Conclusion: Evidence (of moderate quality) suggests that daily administration of empagliflozin 25 mg tablets in adjunct to insulin in T1DM patients with optimum kidney functioning is useful to achieve glycemic control compared to placebo and insulin therapy. However, the effect on blood pressure remained indistinguishable between the compared interventions.

Primary neuroendocrine carcinomas of the urinary bladder are rare. A 60-year-old male presented with gross hematuria for the past 3 months. Diagnostic flexible cystoscopy revealed a papillary lesion above the right ureteric orifice. Transurethral resection of bladder tumor was performed and resected tissue was sent for histopathology that revealed high-grade urothelial carcinoma with small-cell neuroendocrine differentiation. Lamina propria, muscularis propria, and perineural invasion was seen which was later also confirmed by immunohistochemistry. The patient received neoadjuvant four cycles of chemotherapy and then underwent radical cystoprostatectomy with ileal conduit. The patient’s recovery was uneventful and he is on regular follow-up from the past 12 months without any disease recurrence. Early detection and aggressive management can improve the survival and prognosis of these patients.